In Situ Hybridization: A Practical Approach

In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation and function of genes. This popular, hands-on guide has been thoroughly updated to cover the key techniques currently in use. These include in situ hybridization to mRNA with oligonucleotide and RNA probes (both radio labelled and hapten labelled); analysis with light and electron microscopes; whole mount in situ h…